연구분야
Rapid Method for Determination of Capsaicin and Dihydrocapsaicin in Gochujang Using Ultra-High-Perfo
- 작성일2017.05.16 00:00
- 분류
- 조회수2,790
- 링크https://doi.org/10.1093/jaoac/93.6.1905
- 저널명Journal of Aoac International
- 저자하재호,서혜영,심유신,남현진,석호문,Masahito Ito Hiroaki Nakagawa
- 구분
- 유형
제목
Rapid Method for Determination of Capsaicin and Dihydrocapsaicin in Gochujang Using Ultra-High-Performance Liquid Chromatography
요약
A sensitive and specific heating block method coupled with ultra-HPLC (u-HPLC) was developed for the analysis of capsaicin in Gochujang and validated by comparing with a conventional HPLC (AOAC Method 995.03). The method validation parameters yielded good results, including linearity, precision, accuracy, and recovery. The u-HPLC separation was performed on a reversed C18 column (50 ´ 2 mm id, particle size 2 mm), followed by fluorescence detection (excitation 280 nm, emission 325 nm). Methanol was used as the extracting solvent, and the amount of sample taken was approximately 0.2 g; the optimum amount of extraction solvent and extraction time were 15 mL and 1 h, respectively. The recovery of capsaicin in Gochujang was more than 93%, and the LOD and LOQ of the u-HPLC analysis were 0.05 and 0.16 mg/g for capsaicin and 0.05 and 0.16 mg/g for dihydrocapsaicin. The calibration graphs for capsaicin and dihydrocapsaicin were linear from 0.2 to 10.0 mg/mL for u-HPLC. The interday and intraday precisions (RSD values) were <6.27%.
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