연구분야
Nematicidal Activity of Kojic Acid Produced by Aspergillus oryzae against Meloidogyne incognita
- 작성일2017.05.15 00:00
- 분류
- 조회수1,854
- 링크http://www.jmb.or.kr/submission/Journal/026/JMB026-08-06_FDOC_1.pdf
- 저널명Journal of Microbiology and Biotechnology
- 저자김태윤,김진철,장자영,전선정,이혜원,배창환,여주홍,이향범,김인선,박해웅
- 구분
- 유형
제목
Nematicidal Activity of Kojic Acid Produced by Aspergillus oryzae against Meloidogyne incognita
요약
The fungal strain EML-DML3PNa1 isolated from leaf of white dogwood (Cornus alba L.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. The active fungal strain was identified as Aspergillus oryzae, which belongs to section Flavi, based on the morphological characteristics and sequence analysis of the ITS rDNA, calmodulin (CaM), and β-tubulin (BenA) genes. The strain reduced the pH value to 5.62 after 7 days of incubation. Organic acid analysis revealed the presence of citric acid (515.0 mg/kg), malic acid (506.6 mg/kg), and fumaric acid (21.7 mg/kg). The three organic acids showed moderate nematicidal activities, but the mixture of citric acid, malic acid, and fumaric acid did not exhibit the full nematicidal activity of the culture filtrate of EML- DML3PNa1. Bioassay-guided fractionation coupled with (1)H- and (13)C-NMR and EI-MS analyses led to identification of kojic acid as the major nematicidal metabolite. Kojic acid exhibited dose-dependent mortality and inhibited the hatchability of M. incognita, showing EC50 values of 195.2 μg/ml and 238.3 μg/ml, respectively, at 72 h postexposure. These results suggest that A. oryzae EML-DML3PNa1 and kojic acid have potential as a biological control agent against M. incognita.
콘텐츠 정보책임자
- 담당부서 사업예산실
- 담당자 최지석
- 연락처 062-610-1710