The super cooling prechilling method according to the present invention looks at the excellent effect on lactobacillus improved productivity and survival after the freezing of the lactobacillus or the freeze-drying and the storage-stable according to the preservation period shows the excellent effect. Therefore, using the survival rate and storage stability enhancement method of the lactobacillus according to the present invention, the lactobacillus containing product quality can be ultimately improved and the distribution cost of the lactobacillus containing products can be saved by the markedly with the promotion of the storage-stable.

The present invention relates to the kimchi spice mixing device which can mass-produce the high quality kimchi in which the taste and quality are constant by evenly mixing the salted cabbage and spice in the spice mixing box of the dual chamber structure.

As the probiotics in which Lactobacillus sp. WiKim 0092 according to the present invention has the antibacterial activity about the noxiousness pathogen (Clostridioides difficile), it can be variously used for the use including prevention and treatment of the noxiousness pathogen infection disease of human or animal, the seminal plasma etc.

As the probiotics in which Lactobacillus sp. WiKim 0093 according to the present invention has the antibacterial activity about the noxiousness pathogen (Clostridioides difficile), it can be variously used for the use including prevention and treatment of the noxiousness pathogen infection disease of human or animal, the seminal plasma etc.

Lactobacillus fermentum WiKim0102, and the accession number KCCM12356P) is separated from the Kimchi in the active ingredient. The anticancer activity of WiKim0102 is excellent. Therefore it can be usefully used as the composition for the use including the treatment of the cancer of human or animal, the prevention or the improvement etc.

The present invention relates to the Lactobacillus plantarum WiKim0060 which is separated from the Kimchi and is novel and the composition including the same. As the probiotics in which WiKim0060 has melanin and erythema formation inhibition, and the hard husk trans epidermis water loss reduction activity, it can be variously used for the use including prevention and improvement of the skin fruit colour plasticity disease of human or animal, the seminal plasma etc.

Weissella hellenica WiKim0103 is separated from the Kimchi and is novel and the composition including the same. WiKim0103 inhibits fat accumulation in hepatocytes and reduces the expression of fatty liver-related genes, thereby exhibiting an inhibitory effect on fatty liver production. For this reasons, it can be used for various purposes for the prevention, improvement and treatment of obesity or fatty liver disease.

Through the present invention, a calcium-enriched concentrate in which a large amount of ionized calcium is concentrated can be prepared. In addition, it is possible to improve the calcium utilization rate, so it can be applied to food fields such as kimchi.

The present invention relates to a bacteriocin produced from a new Lactobacillus brevis WiKim0069 strain, a functional food containing the same, a natural preservative and an antibacterial agent. The bacteriocin produced from WiKim0069 strain has a different sequence from the existing bacteriocin derived from lactic acid bacteria. It has antimicrobial properties against lactic acid bacteria, and at the same time, has excellent efficacy in both acid resistance and heat resistance, so it can be usefully used as a functional food, natural preservative, and antibacterial agent.

The hydrogel according to the present invention is characterized in that it is possible to increase the content of various physiologically active substances including sulfur in sprout vegetables without supply of nutrient solution, and to prevent contamination of soil or water due to nutrient solution by not supplying nutrient solution. The sprout vegetables grown using hydrogel can be stably dissolved in a solution by powdering and coating treatment, and thus can be usefully used in various foods.

Enterococcus lactis significantly suppresses the expression of MMP-1 and MMP-9 enhanced by ultraviolet irradiation, and relaxes the stratum corneum to reduce skin thickness. In addition, it enhances the amount of moisture in the skin, reduces the amount of transdermal moisture loss, and enhances the content of hyaluronic acid. Therefore, the composition of the present invention can be used as a cosmetic composition for preventing or treating skin wrinkles or skin moisturizing cosmetic, health functional food, feed, veterinary composition, skin dryness.