제목

Cloning and expression of γ-glutamyl transpeptidase and its relationship to greening in crushed garlic (Allium sativum) cloves

요약

BACKGROUND: Garlic greening occurs when garlic cloves are stored at low temperature, increasing 1-propenyl cysteine sulfoxide,whichisinducedbyγ-glutamyltranspeptidase(GGT)activity.Althoughthemetabolismoftheγ-glutamylpeptideis importantforthebiosynthesisofgreenpigmentsincrushedgarliccloves,garlicGGTispoorlycharacterised.
RESULTS:FortheanalysisofGGTatthegenelevel,thegarlicGGT sequencewaspartiallyclonedusinganonionGGT sequence. The relationship between garlic greening and related gene expressions, depending on storage condition, was investigated usingreversetranionpolymerasechainreactionforgarlicGGT andalliinase.Threestorageconditionswereset:A,storage at a constant temperature of 20?C; B, storage at 20?C for 3months and then transfer to 0?C for an additional 3months; C, storage at 0?C for 3months and then transfer to 20?C for an additional 3months. GGT expression increased under storage conditionBanddecreasedunderstorageconditionC.However,alliinaseexpressionwasnotaffectedbystoragecondition.